How to Check Your Intonation in Five Minutes
Your guitar can read perfectly in tune on the open strings and still sound off once you start playing up the neck. That mismatch is an intonation problem, and checking it takes about five minutes with a tuner you probably already own.
What Intonation Actually Means
Intonation is whether every fretted note on the neck matches its true pitch, not just the open strings. Steel strings get slightly stiffer as they are pressed down, which pushes the fretted pitch a touch sharp compared to the open string, and that effect grows the further up the neck you go. The bridge saddle exists to compensate for this by adjusting the string's effective length. When a saddle is set correctly, the guitar stays in tune with itself all the way up the neck. When it is off, you can tune the open strings perfectly and still hear chords turn sour by the twelfth fret.
The Five-Minute Check
Plug in a tuner, a clip-on or pedal tuner both work, and play the twelfth fret harmonic on a string, then compare it to the fretted note at the same fret. If the fretted note reads sharp compared to the harmonic, the saddle needs to move back, away from the neck, to lengthen the string slightly. If it reads flat, move the saddle forward, toward the neck. Make a small adjustment, retune the open string, and check again. Repeat for each string, and the whole process takes about five minutes once you have done it a time or two.

The Right Tool for Your Bridge
Strat-style bridges with six individual saddles usually take a small Phillips or flathead screwdriver turned through the back of the saddle to move it forward or back. Tune-o-matic bridges, common on Gibson-style guitars, use a flathead screwdriver on each saddle screw, and some locking versions need a small hex wrench instead. Either way, a few careful turns is all it takes, so work in small increments and recheck the tuner after each one.

Why It Drifts in the First Place
Old strings lose elasticity and stop reading consistently on a tuner, which is one of the most common reasons intonation seems to wander over time. Switching to a different string gauge changes the tension on the saddle and usually calls for a fresh intonation check, and big swings in temperature or humidity can shift a wooden neck enough to throw things off too. If intonation keeps drifting no matter what you do, worn frets or a truss rod issue further up the neck are worth ruling out.
How Often to Check It
A good habit is to check intonation with every string change, any time you switch string gauge, and after a significant change in temperature or humidity. Regular players often build it into a monthly setup routine, though there is no strict rule since it really depends on how much a particular guitar moves with its environment.
Sources: Intonation fundamentals and the twelfth-fret harmonic method via Fender's Intonation 101 guide and Sweetwater InSync; saddle adjustment tools and technique via StewMac and Ultimate Guitar's Tune-o-matic setup guide; string gauge and intonation relationship via Sweetwater InSync; tuner accuracy specs via TC Electronic and Boss product listings.

